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  • تهران - خيابان شريعتی – خيابان وحيد دستگردی (ظفر)- نرسيده به خيابان دکتر مصدق (نفت سابق) پلاك 174

معرفی بخش

 

هماتولوژی شاخه ای از پزشکی است که به مطالعه، تشخیص، درمان و پیشگیری از بیماری های مربوط به بافت خون می پردازد و ارتباط نزدیکی با رشته های مختلف پزشکی و علوم پایه از جمله: ایمنی شناسی، ژنتیک پزشکی و ژنتیک مولکولی دارد. با توجه به اینکه شمارش سلولی دقیق دستیابی به تشخیص صحیح را تسریع می کند از این رو انجام کلیه شمارش های سلولی با بهترین تکنیک ها و روش های ممکن، یک ضرورت است.

بخصوص این امر در مورد تعیین شمارش پلاکتی و تعیین اندیس های RBC  صادق است. بخش هماتولوژی آزمایشگاه پیوند با انجام کلیه اصول کنترل کیفی، استفاده از جدیدترین و دقیق ترین تجهیزات و دارا بودن کادر مجرب در رابطه با طیف وسیعی از آزمایش های هماتولوژی در حال ارائه خدمت به بیماران و پزشکان محترم می باشد.

 

برخی از شاخصه های بخش هماتولوژی آزمایشگاه پیوند عبارتند از:

  • استقرار سیستم مدیریت کیفیت در قالب استانداردهای ملی و ایزو (10002 +ISO 9001)
  • مجهز بودن به آخرین تکنولوژی روز دنیا و مورد تایید FDA برای شمارش و تشخیص پارامترهای هماتولوژیک
  • قابلیت گزارش بیش از 30 پارامتر در قالب آزمایش CBC به همراه مشاهده ی گستره خون محیطی توسط افراد با تجربه
  • قابلیت ارزیابی و شمارش مایعات بدن از جمله مایع مغزی نخاعی توسط سیستم های اتوماتیک
  • آماده شدن اکثر نتایج بخش به طور روزانه و در موارد اورژانس در عرض 1 الی 2 ساعت
  • بهره گیری از کارکنان با تجربه و دارای حداقل مدرک تحصیلی کارشناسی

 

فهرست آزمایش ها

                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                

CBC & Body Fluids automated cell counting with Siemens ADVIA 2120i/Mindray 6800

fully automated six part diff. (FDA approved)

 

Test Name Assay Method Sample Type Preparation Before
Specimen Collection
Sample Volume Storage Transport Testing Time Reporting Time Rejection Others
ABO Blood Group and Rh (including Du test) Agglutination Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed and Clotted Specimen -
Alkaline Phosphatase (ALK) Staining Cytochemistry Whole Blood / Bone Marrow or Slides
(Heparin Green Cap / PB/BM Unfixed Slides)
N/A 3 mL  Blood: at 2-8°c
Slides: at 20-25°c
Blood: at 2-8°c
Slides: at 20-25°c
Daily Same Day Hemolyzed, Old & Clotted specimen,
EDTA-Anticoagulated Specimen
-
Auto-Hemolysis Test Hemolysis Whole Blood in EDTA Lavender Cap No Blood Transfusion
for 2-3 Weeks Prior to Test
6 mL at 2-8°c at 2-8°c Daily
(except Wednesdays)
in 2 Working Day Hemolyzed, Clotted or Low-Volume Specimen -
Automated ESR Automated Whole Blood in Black Cap (3.2% Sodium Citrate) N/A 2 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Clotted or Low-Volume Specimen -
Automated Retic Count
(including: IRF, CHr, MCVr, #/% retic)
Flow cytometry by cell counter Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Body Fluid Analyses Manual and Advia 2120i (CSF,  pleural, ascites) Body Fluids (CSF, Synovial, Pleural, Ascites, etc)
in Sterile Universal Container
N/A 3-5 mL at 2-8°c at 2-8°c Daily Same Day Old Specimen Fully automated evaluation of body fluids with the Advia 2120i and cytocentrifuged smear examination.
RPMI medium is recommended.
CBC
(by fully automated cell counter)
Mindray BC6800 & Advia 2120i Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Cold Agglutinin Test Agglutination Serum in Clot Red Cap Syringe & vials should be incubated in 37 ºc 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed or Low-Volume Specimen Serum or plasma, separated from a sample maintained at 37°c, or allowed to clot at 37°c
or plasma separated from an anticoagulated sample after periodic inversion at 37°c for approximately 15 min
Coomb's Test - Direct Agglutination Whole Blood in EDTA Lavender Cap N/A 3 mL at Room Temperature at Room Temperature Daily Same Day Old, Clotted or Low-Volume Specimen -
Coomb's Test- Indirect Agglutination Serum in Clot Red Cap N/A  3 mL at 2-8°c at 2-8°c (for 24 hrs.) or Frozen at -20°c Daily Same Day Hemolyzed or Low-Volume Specimen -
Ham’s Test for PNH Hemolysis Patient: Whole blood
Cohort: ABO-Compatible Fresh Serum in EDTA Lavender Cap
No Blood Transfusion
for 2-3 Weeks Prior to Test
6 mL at 2-8°c at 2-8°c Daily in 1 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Heat Instability Test and
Isopropanol Precipitation for Unstable Hbs
Precipitation Whole Blood in EDTA Lavender Cap No Blood Transfusion
for 2-3 Weeks Prior to Test
6 mL at 2-8°c at 2-8°c Daily in 2 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Heinz Body Staining Vital Stain Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily in 1 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Iron (Fe) Staining Cytochemistry Whole Blood / Bone Marrow or Slides
(EDTA Lavender Cap / PB / BM Unfixed Slides)
N/A 3 mL  Blood: at 2-8°c
Slides: at 20-25°c
Blood: at 2-8°c
Slides: at 20-25°c
Daily Same Day Hemolyzed, Old & Clotted specimen -
Isohemagglutinin Test Agglutination Serum in Clot Red Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed or Low-Volume Specimen -
Kleihauer-Betke (KB) for F-Cell%
in Abnormal Hemoglobin syndrome and Fetomaternal Hemorrhage
Acid-Elution Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily in 3 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Lamellar Body Count in Aminiotic Fluid Advia 2120i Aminiotic Fluid in Universal Container N/A 3-5 mL at 2-8°c at 2-8°c Daily Same Day Sample must be without any blood, mucos and meconium.
Sample should not be freezed.
-
LE Cell Test Microscopic Whole De-Fibrinated Blood N/A 3 mL at 2-8°c at 2-8°c Daily in 1 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Malaria/Borrelia Thin and Thick Smear Microscopic Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Manual Retic Count Vital Stain / Microscopic Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Myeloperoxidase (MPO) Staining Cytochemistry Whole Blood / Bone Marrow or Slides
(EDTA Lavender Cap / PB/ BM Unfixed Slides)
N/A 3 mL  Blood: at 2-8°c
Slides: at 20-25°c
Blood: at 2-8°c
Slides: at 20-25°c
Daily Same Day Hemolyzed, Old & Clotted specimen -
NBT Oxidation  EDTA Not Recommended 3 ml at 20-25°c at 20-25°c Daily Same Day MORE THAN 5 HRS. OR NOTROPHIL COUNT LESS THAN 200 Not Recommended
Osmatic Fragility Test  (OFT)
Before/After Incubation with Osmograph
Hemolysis Whole Blood in EDTA Lavender Cap No Blood Transfusion
for 2-3 Weeks Prior to Test
3 mL at 2-8°c at 2-8°c Daily
(except Thursdays)
in 1 Working Day Hemolyzed, Old, Clotted or Low-Volume Specimen,
or Specimen received 6 hrs. after collection
-
PBS Staining for
Golf Ball-Appearing RBC (HbH disease)
Vital Stain Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed and Clotted Specimen -
Periodic Acid Schiff (PAS) Staining Cytochemistry Whole Blood / Bone Marrow or Slides
(EDTA Lavender Cap / PB/ BM Unfixed Slides)
N/A 3 mL Blood: at 2-8°c
Slides: at 20-25°c
Blood: at 2-8°c
Slides: at 20-25°c
Daily Same Day Hemolyzed, Old & Clotted specimen -
Sickle Cell Screening Test
(Metabisulfite Test)
Metabisulfite / Microscopic Whole Blood in EDTA Lavender Cap N/A 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Sucrose Haemolysis Test for PNH Hemolysis Patient: Whole Blood and Fresh Serum in EDTA Lavender Cap
Cohort: ABO-Compatible Fresh Serum in Clot Red Cap
No Blood Transfusion
for 2-3 Weeks Prior to Test
 3 mL at 2-8°c at 2-8°c Daily Same Day Hemolyzed, Old, Clotted or Low-Volume Specimen -
Tartrate-Resistant Acid Phosphatase (TRAP) Staining Cytochemistry Whole Blood / Bone Marrow or Slides
(EDTA Lavender Cap / PB / BM Unfixed Slides)
N/A 3 mL Blood: at 2-8°c
Slides: at 20-25°c
Blood: at 2-8°c
Slides: at 20-25°c
Daily Same Day Hemolyzed, Old & Clotted specimen -

**Full Automated evaluation of body fluids with the Advia 2120 and cytocentrifuged smear exam.

*serum or plasma, separated at 37̊ C from a sample maintained /or allowed to clot at 37̊ C or plasma, separated from an anticoagulated sample after periodic inversion at 37 ̊ C for approximately 15 min.

 

 

 

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