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معرفی بخش

(FISH (Flourescent In Situ Hybridization تکنیکی است که در حوزه علم ژنتیک بررسی و شناسایی مکان یابی ژن ها یا توالی DNA و RNA را بصورت مستقیم بر روی کروموزوم امکان پذیر ساخته است. که در این راستا به منظور کاوش، توالی اختصاصی با مواد فلورسنت نشاندار می گردند. از جمله مزیت های این تکنیک در علم بیولوژی مولکولی و ژنتیک وسیع الطیف بودن کاربرد تشخیصی آن در بیماری های انکولوژی، هماتولوژی، بیماری های عفونی (ویروسی، باکتریال)، بیماری های ارثی و ژنتیکی می باشد.

بدین منظور آزمایشگاه تشخیص طبی و تخصصی پیوند در راستای تحقق اهداف خود با بهره گیری از ابزار پیشرفته و تکنولوژی روز دنیا و با پشتوانه ی نیروی متخصص و کارامد نسبت به راه اندازی بخش FISH اقدام نموده است تا بتواند سهم کوچکی برای تشخیص بیماری های بخصوصی چون هماتولوژی (CLL، AML، ALL، MPNs، CML)   و انکولوژی (Neuroblastoma , small-cell lung cancer , Breast cancer)  برای کمک به درمان بیماران این مرز و بوم داشته باشد.

به منظور انجام تکنیک FISH در آزمایشگاه تشخیص طبی و تخصصی  پیوند کلیه نمونه های پذیرش شده بخش FISH  بسته به نیاز می تواند فرآیند کشت سولی و هاروست با روش های متداول صورت پذیرد و یا این امر صرفا با محیط کشت پایه انجام شود؛ بعد از آن اقدامات وابسته جهت تهیه لام (4-3 لام) صورت پذیرفته که بعد از دناتوراسیون، هیبریداسیون و انکوباسیون با پروب اختصاصی مربوط به هر ناهنجاری و مراحل شستشو در نهایت با رنگ آمیزی، مطالعه سلولی ( شمارش 200 سلول) در مرحله اینترفاز انجام می شود که با تهیه ده ها عکس نسبت به آنالیز سلولی بر طبق الگوی پروب های مصرفی و تهیه گزارش کتبی به همراه عکس های مصور مربوط به بیماران مراجعه کننده اقدام می شود.

 

فهرست آزمایش ها

                         

 Panel Name Analyte/Investigation Sample Type Preparation before Specimen Collection Sample Volume Storage Transport Testing Time Reporting Time Rejection
AML panel t(15;17)(q24;q21)
PML/RARA for AML-M3
Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
ALL panel Xp22/Yp11 Rearrangement/Deletion  (CRLF2/P2RY8) for ALL Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
t(12;21)(p13;q22) ETV6/RUNX1(iAMP21) for ALL Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
11q23 Rearrangement (MLL) for ALL/AML Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
t(9;22)(q34;q11) ABL1/BCR for CML Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
Breast Cancer 17q12 Amplification (HER2 amp,17cen)  for Breast cancer FFPE Not Recommended FFPE from cancer/tumoral zone: 1pc  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 7-10 working days Poor quality fixation in anything except formalin
Burkitt Lymphoma Panel t(8;14)(q24;q32) c-Myc/IgH
for Burkitt Lymphoma / ALL- L3
Preferred Specimen: Bone Marrow
Whole blood
Alternate:**FFPE
Not Recommended EDTA lavender Cap 3 mL
FFPE from cancer/tumoral zone: 1pc
 Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days Sample lysis-Freeze-have a Clotted
14q32 Rearrangement (IGH) for Burkitt Lymphoma / ALL- L3 Preferred Specimen: Bone Marrow
Whole blood
Alternate:**FFPE
Not Recommended EDTA lavender Cap 3 mL
FFPE from cancer/tumoral zone: 1pc
 Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
8q24 Rearrangement (c-MYC) for Burkitt Lymphoma / ALL- L3 Preferred Specimen: Bone Marrow
Whole blood
Alternate:**FFPE
Not Recommended EDTA lavender Cap 3 mL
FFPE from cancer/tumoral zone: 1pc
 Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
2p11 Rearrangement (IGK) for Burkitt Lymphoma / ALL- L3 Preferred Specimen: Bone Marrow Whole blood
Alternate: FFPE
Not Recommended EDTA lavender Cap 3 mL
FFPE from cancer/tumoral zone: 1pc
 Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days Sample lysis-Freeze-have a Clotted
22q11 Rearrangement (IGL) for Burkitt Lymphoma / ALL- L3 Preferred Specimen: Bone Marrow
Whole blood
Alternate:**FFPE
Not Recommended EDTA lavender Cap 3 mL
FFPE from cancer/tumoral zone: 1pc
 Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
CLL Panel Deletion 17p13 (TP53)
Deletion 11q22 (ATM) for CLL prognosis 
Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
Deletion 13q14 (DLEU)
Deletion 13q34 (LAMP)
Trisomy 12 (12cen) for CLL prognosis
Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days Sample lysis-Freeze-have a Clotted
Glial Brain Tumors Deletion 1p36 Glioma
Deletion 1q25 Glioma
Deletion 19q13 Glioma
Deletion 19p13 Glioma
for Glial Brain Tumors
FFPE Not Recommended FFPE from cancer/tumoral zone: 1pc  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days Poor quality fixation in anything except formalin
MDS Panel Deletion 5q31 (EGR1) for Myelodysplastic syndromes (MDS) Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
Deletion 7q22-31 for Myelodysplastic syndromes (MDS) Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed Saturday through Wednesday  (7a.m - 17 p.m.), Thursday (7a.m - 12 p.m.) in 5-7 working days
Clotted, Freezed, Hemolyzed
Trisomy 8 (8cen) for Myelodysplastic syndromes (MDS) Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
Deletion 20q12-13 for Myelodysplastic syndromes (MDS) Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
Multiple Myeloma Panel t(14;16)(q32;q23) IGH /MAF/WWOX
 for Multiple Myeloma prognosis
Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed
t(4;14)(p16;q32) FGFR3/IGH Whole blood
Bone Marrow
Not Recommended EDTA lavender Cap 3 mL  Do not freeze. Use cold pack for transport, making sure cold pack is not in direct contact with specimen. at 4-8 °c Samples processed
Sat till Wed  (7a.m - 17 p.m.)
Thursday (7a.m - 12 p.m.)
in 5-7 working days
Clotted, Freezed, Hemolyzed

 

 فایل های راهنما

    

نحوه ارسال نمونه خون برای آزمایش­های مولکولی

نحوه ارسال نمونه برای انجام تست FISH خون محیطی و مغز استخوان

                                                                                                                                                                                                                                                                                   

 

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